This invention pertains to BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter operably linked to the DNA segment; and a transgene operably linked to a second promoter; b) a BIV packaging vector construct comprising a BIV DNA sequence fragment comprising at least a <u>gag</u> gene or <u>pol</u> gene of BIV; a promoter operably linked to the BIV DNA fragment; and a polyadenylation sequence located downstream of the BIV DNA fragment; and c) an expression vector construct comprising a gene encoding a viral surface protein. Also provided is a method for transferring a gene of interest into a mammalian cell.
申请公布号
WO0144458(A2)
申请公布日期
2001.06.21
申请号
WO2000US33725
申请日期
2000.12.13
申请人
NOVARTIS AG;LUO, TIANCI;BERKOWITZ, ROBERT, DAVID;KALEKO, MICHAEL
发明人
LUO, TIANCI;BERKOWITZ, ROBERT, DAVID;KALEKO, MICHAEL