METHOD FOR AMPLIFYING NUCLEIC ACID SEQUENCE

摘要

PROBLEM TO BE SOLVED: To provide a method for synthesizing a DNA, particularly for simply and efficiently amplifying a nucleic acid sequence. SOLUTION: This method comprises the following steps: (a) a strand of a nucleic acid sequence to be a template is treated with one oligonucleotide primer or more to synthesize an elongation product of a primer complementary to the strand of the nucleic acid sequence, (b) a double-stranded DNA from 5' end of the primer elongation strand obtained in step (a) is partially denatured to anneal an oligonucleotide primer having a base sequence same as the primer obtained in step (a), and (c) the strand of the nucleic acid sequence complementary to the template is substituted from 3' end of the primer of step (a) is reused in step (b).