发明名称 |
SINGLE-STRANDED OLIGODEOXYNUCLEOTIDE MUTATIONAL VECTORS |
摘要 |
The invention concerns the introduction of predetermined genetic changes in target genes of a living cell by introducing an oligodeoxynucleotide encodin g the predetermined change. The oligodeoxynucleotides are effective in animal, plant and bacterial cells. Specific end modifications that greatly increase the effectiveness of the oligodeoxynucleotides in bacteria are described. Surprisingly, unmodified oligodeoxynucleotides can be as effective in mammalian cells, including in vivo hepatocytes, as the modified nucleotides and can be as effective or more effective than chimeric oligonucleotides tha t consist of a mixture of deoxynucleotides and 2'-O-methyl ribonucleotides.</S DOAB>
|
申请公布号 |
CA2382120(A1) |
申请公布日期 |
2001.03.08 |
申请号 |
CA20002382120 |
申请日期 |
2000.08.25 |
申请人 |
VALIGEN (US), INC. |
发明人 |
METZ, RICHARD A.;FRANK, BRUCE L.;WALTHER, DEBRA M. |
分类号 |
A01K67/027;A61K48/00;A61P43/00;C12N5/10;C12N15/05;C12N15/09;C12N15/10;C12Q1/02;(IPC1-7):A61K48/00;C07H21/04;C12N15/85 |
主分类号 |
A01K67/027 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|