| 摘要 |
The invention relates to a quick method for the selection of active clones for industrial biosynthesis of streptomycin which consists in isolating 10 ... 50 colonies having a typical morphology, subjecting each of them to homogenization by vibrations in 2 ml of distilled water, inoculating 30 ml of biosynthesis medium with 1 ml of suspension, said biosynthesis medium having the following composition : 50 .... 10 g / l glucose, 10 ... 50 g / l soya meal, 50 ... 20 g / l ammonium sulphate, 0.1 ... 0.7 g / l ammonium phosphate, 1 ...5 g / l sodium chloride, 5 ...15 g / l calcium carbonate which is incubated on a rotating mixer having 270 rpm at 27 degree C, for 7 days, afterwards determing the streptomycin concentration, which varies between 5,000 and 1,500 u / ml, simultaneously with inoculating each of the 3 slants with 0.3 ml of the remaining suspension, said slants being incubated at 27 degree C for 12 ... 14 days, afterwards separating those slants originating in the colonies which generated an antibiotic concentration of over 10,000 u / ml per bottle and are used for industrial purposes, without undergoing steps involving the use of the laboratory vegetative matter prepared from slant, and from which the laboratory fermentation is inoculated, eliminating potential culture degradation by increasing the variability and by storing the slant in the refrigerator.
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