发明名称 |
POLYNUCLEOTIDE AMPLIFICATION METHOD |
摘要 |
Methods useful for improving results obtained with enzyme-based polynucleotide amplification reactions. More particularly, the invented methods are useful for: (1) promoting amplification of template-specific products such that the amount of amplicon produced reflects the pre-amplification amount of analyte, even in reactions primed with low levels of analyte polynucleotide; (2) facilitating biological specimen processing such that the amount of amplicon produced in subsequent amplification reactions will be substantially independent of the efficiency of analyte polynucleotide isolation from the specimen; and (3) controlling the amount of analyte amplicon produced in the amplification reaction.
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申请公布号 |
WO0107661(A2) |
申请公布日期 |
2001.02.01 |
申请号 |
WO2000US20034 |
申请日期 |
2000.07.21 |
申请人 |
GEN-PROBE INCORPORATED;NUNOMURA, KIYOTADA |
发明人 |
NUNOMURA, KIYOTADA |
分类号 |
G01N33/53;C12N15/09;C12Q1/68;G01N21/78;G01N33/566;G01N33/58;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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