METHOD FOR SELECTING OLIGONUCLEOTIDES HAVING LOW CROSS-HYBRIDIZATION

摘要

The present invention provides methods to provide a set of probes that hybridize or bind relatively well to their intended targets but that do not substantially bind or hybridize to unintended targets. The quality of hybridizing or binding relatively well to intended targets but not to unintended targets may be quantified using delta Tm according to the methods of the present invention. The present invention also provides sets of probes produced according to the subject methods and programmed computer systems functional to perform the subjects methods.