发明名称 |
Cell-based assay for screening cox-2 inhibitors |
摘要 |
A cell-based assay is provided for screening substances for the capacity to inhibit COX-2 activity. The assay utilizes as a screening reagent a cell line which has been engineered to achieve constitutive or inducible expression of COX-2. In one embodiment, the cells express a GTPase-deficient, constitutively-activated mutant form of the alpha -subunit of protein G12 which induces the production of arachidonic acid and COX-2. Decreased cell proliferation in the presence of test substance indicates that the substance has COX-2 inhibitory activity. |
申请公布号 |
AU5615200(A) |
申请公布日期 |
2001.01.02 |
申请号 |
AU20000056152 |
申请日期 |
2000.06.15 |
申请人 |
TEMPLE UNIVERSITY - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCAT |
发明人 |
E. PREMKUMAR REDDY;M.V. RAMANA REDDY;N. DHANASEKARAN |
分类号 |
C12Q1/26;G01N33/50;G01N33/88 |
主分类号 |
C12Q1/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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