发明名称 |
Method for the high level expression, purification and refolding of the outer membrane protein P2 from Haemophilus influenzae type B |
摘要 |
The present invention relates, in general, to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b (Hib-P2) and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b or fusion protein thereof in E. coli wherein the Hib-P2 protein or fusion protein comprises more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of Hib-P2 protein and fusion protein thereof.
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申请公布号 |
US6153406(A) |
申请公布日期 |
2000.11.28 |
申请号 |
US19930096181 |
申请日期 |
1993.07.23 |
申请人 |
NORTH AMERICAN VACCINE, INC. |
发明人 |
TAI, JOSEPH Y.;PULLEN, JEFFREY K.;SOPER, THOMAS;LIANG, SHU-MEI;BLAKE, MILAN S. |
分类号 |
C12N15/09;A61K39/00;A61K39/102;A61P31/04;C07K1/34;C07K14/01;C07K14/11;C07K14/285;C07K16/10;C12N1/21;C12P21/02;C12P21/04;C12R1/19;C12R1/92;(IPC1-7):C12N15/09;C12N1/20 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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