发明名称 |
METHODS FOR MAKING POLYKETIDES |
摘要 |
The stereochemical centers of a polyketide can be changed by replacement of ketosynthase domains in the polyketide synthase (PKS) enzyme that produces the polyketide. The specificity of the AT domains of a PKS is determined by a hypervariable region that can be replaced or altered to change the specificity of the AT domain from a naturally occurring extender unit to another naturally or non-naturally occurring extender unit. Non-naturally occurring extender units, including methylmalonyl N-acetyl cysteamine thioester can be incorporated into polyketides in recombinant host cells or in cell-free systems to make polyketides. |
申请公布号 |
WO0001838(A3) |
申请公布日期 |
2000.11.16 |
申请号 |
WO1999US15047 |
申请日期 |
1999.07.02 |
申请人 |
THE BOARD OF REGENTS OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
KHOSLA, CHAITAN;LAU, JANICE;POHL, NICOLA, L. |
分类号 |
C12N15/09;C07C327/30;C12N9/10;C12N15/52;C12P19/62 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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