A method of protein removal is provided, which utilizes a protein digesting enzymes and a detergent that is compatible with ultrafiltration. The method is particularly suited for isolating trace amounts of nucleic acid from a solution that has high protein concentration. The recovered nucleic acid is free of protein that may interfere with downstream application such as nucleic acid quantification or diagnostic use. A kit suitable for use in the protein removal method is also provided.
申请公布号
WO0034504(A3)
申请公布日期
2000.10.26
申请号
WO1999US29017
申请日期
1999.12.09
申请人
GENETIC VECTORS, INC.;MCCABE, MEAD, M.;HENDERSON, RAYMOND