PREPARING METHOD FOR RECOMBINANT PROTEIN USING FUSION PARTNER

摘要

PURPOSE: Provided is a method for preparing recombinant protein or peptide. Thereby, high purity of object protein is mass produced in the form of insoluble inclusion body from recombinant E.coli and the inclusion body is soluble in alkali solution without using denaturant and reductant. CONSTITUTION: The recombinant protein is prepared by using XG-L-Y type fusion protein comprising peptide having excellent self-coherence property as a fusion partner, wherein XG is fusion partner, L is a linker and Y is recombinant protein or peptide. The fusion partner is hybrid polypeptide consisting of hetero peptides from human glucagon and human tumor necrecis factor or popypeptide in which only amino acids of human glucagon are repeated up to 3.