A process for amplifying DNA using a thermostable DNA polymerase from Anaerocellum thermophilum characterised in that it works in the presence of magnesium ions and the absence of manganese ions

摘要

A process for amplifying DNA, characterised in that a thermostable DNA polymerase is used that is obtainable from Anaerocellum thermophilum that catalyses the template directed polymerisation of DNA. The polymerase possess 5'-3'-exonuclease activity and a reverse transcriptase activity in the presence of magnesium ions and in the absence of manganese. The polymerase also exhibits a Mg2+ dependent reverse transcriptase activity of more than 30% relative to the DNA polymerase activity which is set to 100%.