LIPASE PURIFIED WITH ORGANIC SOLVENT AND PREPARATION METHOD OF CHIRAL MEDICAL AND PHARMACEUTICAL PRODUCTS USING THE LIPASE

摘要

PURPOSE: Lipase purified with an organic solvent, its preparation method and chiral medical and pharmaceutical products using the lipase are provided, which lipase is improved in the enantiomeric ratio and is useful to prepare optically pure (S)-ketoprofen, (S)-ibuprofen and (S)-suprofen. CONSTITUTION: A lipase with improved enantiomeric ratio is prepared by adding 0.3-1.0 times by volume of an organic solvent to Candida rugosa lipase solution to collect the first-stage precipitate; adding 0.3-1.0 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the second-stage precipitate; and 0.8-1.2 times by volume of an organic solvent based on the volume of the original lipase solution to the supernatant, to collect the precipitate. The organic solvent is acetone or isopropane.