alpha -amylase gene having ability for highly producing maltopentaose, vector containing said gene and transformant

摘要

A modified enzyme having a reduced maltopentaose decomposing activity and improvements in practical usability was provided by a gene coding for alpha -amylase highly producing maltopentaose, the alpha -amylase comprising an amino acid sequence where an amino acid residue at 57- or 139-position has been substituted in the amino acid sequence of maltopentaose-forming amylase derived from Pseudomonas sp. KO-8940 (Shida, O. et al., Biosci. Biotech. Biochem. Vol. 56, 76-80 (1992)).