| 摘要 |
PROBLEM TO BE SOLVED: To obtain a medicine useful as an antitumor agent by including a specific benzopyran derivative and its salt as active ingredients. SOLUTION: This medicine comprises a benzopyran derivative of the formula and its salt as active ingredients. The compound of the formula is obtained by obtaining a methanol extract from propolis ground by a mixer, separating the methanol extract by absorption-based column chromatography using a silica gel column, subjecting the liquid to inclination elution by concentration gradient using methyl chloride and methanol, collecting a fraction having 100-95% methyl chloride concentration, and elution fractions (Rf value: 0.3) of methyl chloride and methanol (in a ratio of 95%:5% v/v) as the developing solvents, dissolving the concentrate in a mixed solution of methanol, or the like, separating the solution by liquid chromatography using an ODS-based column, eluting the fractions with a mixed solution of acetonitrile, irradiating the main fraction with ultraviolet rays, distilling the solvent of the resultant photoisomer fractions away, and further separating the fractions by liquid chromatography using an ODS-based column to carry out elution, or the like. |
