RECOMBINANT BIOADHESIVE PROTEIN ANALOGS COMPRISING HYDROXYPROLINE

摘要

A method for the production of bioadhesive precursor protein analogs having incorporated therein hydroxyproline, wherein vectors comprising DNA sequences encoding the bioadhesive protein precursor analog are constructed and used to transform a microbial host, preferably E. coli. During growth, the host is contacted with a hypertonic media comprising hydroxyproline, resulting in assimilation and incorporation of hydroxyproline into the bioadhesive precursor protein during translation. Incorporation of hydroxyproline into the bioadhesive precursor proteins during protein production is advantageous in that post-translational modification of the proteins are not required.