发明名称 |
Enrichment method for variant proteins with altered binding properties |
摘要 |
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
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申请公布号 |
US6040136(A) |
申请公布日期 |
2000.03.21 |
申请号 |
US19970923854 |
申请日期 |
1997.09.03 |
申请人 |
GENENTECH, INC. |
发明人 |
GARRARD, LISA J.;HENNER, DENNIS J.;BASS, STEVEN;GREENE, RONALD;LOWMAN, HENRY B.;WELLS, JAMES A.;MATTHEWS, DAVID J. |
分类号 |
A61K47/48;C07K14/01;C07K14/47;C07K14/575;C07K14/61;C07K19/00;C12N5/10;C12N15/10;C12N15/11;C12N15/62;C12N15/85;C12Q1/68;C40B40/02;G01N33/554;G01N33/566;G01N33/74;(IPC1-7):C12Q1/70;C12P21/06;C07K14/00;C07H17/00 |
主分类号 |
A61K47/48 |
代理机构 |
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代理人 |
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地址 |
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