| 摘要 |
The invention concerns a purified recombinant glycoprotein having the following properties: a) an adherence capacity to CD4; b) an affinity with a anti-gp120 antibody capable of neutralising in vitro HIV cell infection; c) an affinity with an anti-gp41 antibody; d) a trimeric form free from inter-chai n disulphide bonds. The invention also concerns a vaccine comprising said purified glycoprotein and an adjuvant. The invention further concerns a meth od for obtaining said glycoprotein as per Claim 1, which consists in expressing , by means of genetic recombining techniques, a glycoprotein corresponding to the properties a), b) and c) mentioned in Claim 1; purifying it, and subjecting it to steps involving at least a reducing agent, an ionic deterge nt and/or a neutral detergent in conditions leading to a glycoprotein having sa id properties.
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