| 摘要 |
PCT No. PCT/CA96/00837 Sec. 371 Date Jun. 19, 1998 Sec. 102(e) Date Jun. 19, 1998 PCT Filed Dec. 13, 1996 PCT Pub. No. WO97/23497 PCT Pub. Date Jul. 3, 1997A linker arm for solid support oligonucleotide synthesis, the linker arm comprising formula (1), wherein: X1 is selected from the group consisting of -O-, -S-, -S(O)2-, -C(O)- and -N(R12)-; R12 is selected from the group comprising hydrogen, a substituted or unsubstituted C1-C20 alkyl group, a substituted or unsubstituted C5-C30 aryl group and a substituted or unsubstituted C5-C40 alkaryl group, X3 is -O- or -N(H)-; R1, R2, R3, R4 and R5 are the same or different and are selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C1-C20 alkyl group, a substituted or unsubstituted C5-C30 aryl group and a substituted or unsubstituted C5-C40 alkylaryl group; n is 0, 1 or 2; and one of A' and B' is selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C1-C20 alkyl group, a substituted or unsubstituted C5-C30 aryl group and a substituted or unsubstituted C5-C40 alkylaryl group, and the other of A' and B' has formula (2), wherein X2 is selected from the group consisting of -O-, -S-, -S(O)2- and -N(R13)-; R13 is selected from the group comprising hydrogen, a substituted or unsubstituted C1-C20 alkyl group, a substituted or unsubstituted C5-C30 aryl group and a substituted or unsubstituted C5-C40 alkaryl group; R6 and R7 are the same or different and are selected from the group consisting of hydrogen, halide, a substituted or unsubstituted C1-C20 alkyl group, a substituted or unsubstituted C5-C30 aryl group and a substituted or unsubstituted C5-C40 alkylaryl group; p is 0 or 1; and m is 0, 1 or 2. A process for producing the linker arm is also disclosed. The linker arm is useful in solid support oligonucleotide synthesis and is characterized by a desirable combination of stability against spontaneous hydrolysis and ease of intentional cleavage of the synthesized oligonucleotide from the linker arm.
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