mutant form of 2,5-diketo-D-gluconic acid reductase A and B enzymes to produce 2-keto-L-gluconic acid (a vit C precursor) by site directed mutagenesis

摘要

A mutant form of 2,5-DKG reductase A that has an amino acid sequence that differs from the wild form of 2,5-DKG reductase A by having an amino acid substitution at position 22 or position 272 and thereby having an improved ability to convert 2,5-DKG to 2-KLG is claimed.