发明名称 |
VERFAHREN ZUR HERSTELLUNG VON PROTEASE |
摘要 |
Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group, in an aqueous medium in the presence of a protease to result a peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from an organic solvent suspension or dissolving said addition compound by adding a water immiscible organic solvent and separating the organic solvent phase by a liquid-liquid separation. The addition compound can be dissolved in an organic solvent after separating the precipitate from the reaction mixture. The addition compound can be also dissolved by adding a water-immiscible organic solvent to the reaction mixture and the organic solvent phase can be separated from the aqueous phase. |
申请公布号 |
DE2822528(A1) |
申请公布日期 |
1978.12.07 |
申请号 |
DE19782822528 |
申请日期 |
1978.05.23 |
申请人 |
TOYO SODA MANUFACTURING CO.,LTD.;YAMAGUCHI(ZAIDANHOJIN)SAGAMI CHEMICAL RESEARCH CENTER |
发明人 |
NONAKA,YUJI;OYAMA,KIYOTAKA;SATOH,HEIJIRO |
分类号 |
C12N9/00;C07K5/06;C07K5/075;C12N9/50;C12P21/02 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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