发明名称 |
DNAs encoding new fusion proteins and processes for preparing useful polypeptides through expression of the DNAs |
摘要 |
<p>This invention relates to a DNA comprising a nucleotide sequence encoding a fusion protein, wherein the fusion protein comprises: a sequence of a signal peptide of a Bacillus cell wall protein (CWP) ; a tag sequence for separation and purification of the fusion protein; a linker sequence; a sequence for chemical or enzymatic cleavage; and an exogenous polypeptide sequence, said sequences being linked in order, said signal peptide, tag and linker being optional sequences. The nucleotide sequence encoding a fusion protein is ligated to the 3'-end of a nucleic acid sequence comprising a Bacillus promoter region. The invention also relates to a vector comprising the DNA; to a bacterium belonging to the genus Bacillus comprising the vector; and to a process for preparation of a useful polypeptide by culture of the bacterium. <IMAGE></p> |
申请公布号 |
EP0955370(A2) |
申请公布日期 |
1999.11.10 |
申请号 |
EP19990302514 |
申请日期 |
1999.03.31 |
申请人 |
ITOHAM FOODS INC.;UDAKA, SHIGEZO |
发明人 |
SATO, SEIJI;HIGASHIKUNI, NAOHIKO;KUDO, TOSHIYUKI;KONDO, MASAAKII |
分类号 |
C07K14/605;C07K14/61;C07K14/62;C07K14/655;C12N15/62;(IPC1-7):C12N15/62;C12N15/17;C12N15/16;C12N15/18 |
主分类号 |
C07K14/605 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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