| 摘要 |
PROBLEM TO BE SOLVED: To obtain fine particles of high purity polypeptide in high recovery rate without being accompanied by any degeneration by adding a specific organic solvent after lyophilizing a mixed aqueous solution of polypeptide and polyethylene glycol. SOLUTION: The subject fine particles preferably having average particle size <=10μm is obtained by adding the organic solvent (C) in which the constituent (A) is insoluble but the constituent (B) is soluble, after lyophilizing the mixed aqueous solution of polypeptide (A) preferably having molecular weight of 1,000 to 200,000 more preferably, 5,000 to 70,000 and polyethylene glycol (B) of molecular weight; 400 to 500,000 more preferably 2,000 to 100,000. Hormones or enzymes or cytokines or growth promoting substances are cited as the constituents (A). Methylene chloride or acetone or ethyl acetate or ethanol or the like are cited as the constituents (C). The preferable concentration ratio of the constituents (B)/(A) is more than the phase inversion point, such as >=0.25.
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