发明名称 |
Detection of mutation by resolvase cleavage |
摘要 |
Methods are disclosed for detecting one or more mutations in an isolated test nucleic acid by forming a heteroduplex with a homologous control DNA and contacting the heteroduplex with a resolvase capable of recognizing at least one single base pair mismatch within the heteroduplex. In preferred embodiments of the invention, the resolvase is bacteriophage T4 endonuclease VII.
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申请公布号 |
US5958692(A) |
申请公布日期 |
1999.09.28 |
申请号 |
US19970922169 |
申请日期 |
1997.09.02 |
申请人 |
VARIAGENICS, INC. |
发明人 |
COTTON, RICHARD G. H.;YOUIL, RIMA;KEMPER, BORRIES W. |
分类号 |
G01N33/50;C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C07H21/02;C07H21/04 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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