MASS SPECTROMETRIC METHODS FOR SEQUENCING NUCLEIC ACIDS

摘要

A mass spectrometric method for sequencing nucleic acids using RNA polymerases, including DNA-dependent and RNA-dependent RNA polymerases, is provided. The methods include use the RNA polymerase is to generate a set of nested RNA transcripts, which are analyzed mass spectrometry. A method of identifying transcriptional terminator sequences or attenuator sequences is also provided.