发明名称 |
HUMAN RIBONUCLEASE A WITH REDUCED RIBONUCLEASE INHIBITOR AFFINITY |
摘要 |
Modified ribonucleases belonging to the RNase A superfamily of ribonucleases is disclosed. Each modified ribonuclease has a mutation in the loop region corresponding to amino acids 85-94 of bovine pancreatic RNase A. Each modified ribonuclease has reduced binding affinity for ribonuclease inhibitor (RI), wild-type ribonuclease activity, and exhibits enhanced cytotoxicity toward tumor cells, relative to the wild-type ribonuclease. Also disclosed is a method for obtaining a modified ribonuclease having reduced binding affinity for RI, wild-type ribonuclease activity, and enhanced cytotoxicity.
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申请公布号 |
WO9919494(A1) |
申请公布日期 |
1999.04.22 |
申请号 |
WO1998US21545 |
申请日期 |
1998.10.13 |
申请人 |
WISCONSIN ALUMNI RESEARCH FOUNDATION |
发明人 |
RAINES, RONALD, T.;LELAND, PETER, A.;SCHULTZ, L., WAYNE |
分类号 |
C12N15/09;A61K38/00;A61K38/46;A61P35/00;A61P43/00;C12N9/22;C12N15/55;(IPC1-7):C12N15/55 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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