发明名称 |
METHOD FOR SYNTHESISING AND AMPLIFYING NUCLEIC ACIDS |
摘要 |
The invention relates to a method for synthesising and amplifying nucleic acids by means of an enzyme-catalysed reaction wherein a nucleic acid is copied on the basis of a single-stranded initiator nucleic acid without said initiator nucleic acid hybridising with the nucleic acid being copied. According to the inventive method, a linear nucleic acid is copied on the basis of an initiator nucleic acid with a single-stranded area at least at the 3' end, through the activity of a polymerase. The initiator nucleic acid does not need to be homologous with the nucleic acid being copied. |
申请公布号 |
WO9915698(A2) |
申请公布日期 |
1999.04.01 |
申请号 |
WO1998EP06006 |
申请日期 |
1998.09.21 |
申请人 |
INSTITUT FUER MOLEKULARE BIOTECHNOLOGIE E.V.;ELLINGER, THOMAS;EHRICHT, RALF |
发明人 |
ELLINGER, THOMAS;EHRICHT, RALF |
分类号 |
C12N15/09;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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