| 摘要 |
PROBLEM TO BE SOLVED: To obtain a highly efficient compact detector for detecting fluorescence in real time by arranging a plurality of tubular migration means, a set of four distributed refractive index lenses, and band filters having different transmission region in the same number as the migration means. SOLUTION: The hollow part of a glass tube 1 in a tubular migration means is filled with a polymer of migration medium and a sample labeled with fluorescent pigments of tour colors is set in the glass tube 1 to cause electrophoresis. The sample is irradiated with fluorescence excitation laser at a position in the glass tube 1 of a given migration length. Fluorescence is emitted from the fluorescence labeled sample when it is irradiated with an exciting light. The fluorescence is taken through four distributed refractive index lenses 9a-9d, as a parallel light, into a detection system. When the fluorescent image is focused tightly through the distributed refractive index lenses, a uniform signal level can be obtained between the center and the end and the base length can be read out similarly to the vicinity of the center. The parallel light is subjected to spectroscopy through band-pass filters 11a-11d and detected by a two-dimensional CCD 13. |
