Heterologous expression of proteins by rescued vector comprising an intron

摘要

A nucleic acid expression construct comprising: (a) a promoter; (b) an intron whose natural position is within the 5'-untranslated region of a gene from which it is derived; (c) a coding sequence; and (d) a 3'-flanking sequence wherein the intron (b) is not derived from the same gene as that from which either the promoter (a) or the protein-coding sequence (c) is derived and processes, vectors, hosts and uses involving such a construct to obtain inter alia an increase in the level of expression of the coding sequence.