Process for obtaining ribosome inactivator proteins known as beetin 27 and beetin 29 from the leaves of Beta vulgaris (sugarbeet) infected with yellow virus

摘要

Process for obtaining ribosome inactivator proteins known as beetin 27 and beetin 29 from the leaves of Beta vulgaris (sugarbeet) infected with yellow virus. A process for obtaining two ribosome inactivator proteins known as beetins 27 and 29 from the plant Beta vulgaris from an extract obtained from the intracellular liquid of the leaves of Beta vulgaris infected with virus and not from an extract of the total plant is described. The general process for obtaining beetins according to this invention includes the stages indicated below: a) Obtaining the extract of the intercellular liquid from the leaves of the said plant. b) Dialysis and development of an ion exchange chromatograph of the protein obtained in the previous stage with a salt gradient. c) Molecular exclusion chromatography of the protein peaks from the previous stage. The said proteins have application as inhibitors in the biosythesis of proteins and as virus genome ribonucleic acid inactivators and are useful in the treatment of cancer and AIDS and viral plant diseases.