IMPROVED METHOD FOR ISOLATING AND RECOVERING TARGET DNA OR RNA MOLECULES HAVING A DESIRED NUCLEOTIDE SEQUENCE

摘要

The invention generally concerns the use of amino acid denaturants for denaturing or separating double standed nucleic acid molecules. More specifically, the present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of haptenylated probes and amino acid denaturants to select the desired molecules and eliminate the undesired library members from a sample. The invention also provides a method in which larger or full-length nucleic acid molecules can be isolated from the subpopulation of desired molecules.