发明名称 |
MODIFIED PHEUMOLYSINE RECOMBINANT PLASMID, METHOD OF PRODUCING MODIFIED PHEUMOLYSINE AND VACCINE |
摘要 |
FIELD: gene engineering, more particularly preparation of modified pneumolysine and manufacture of vaccine based thereon. SUBSTANCE: modified pneumolysine is produced by oligonucleotide directed mutagenesis. Modified wild-type pneumolysine gene is then expressed in bacteria E.Coli. Modified pneumolysine has certain amino acid sequence. One or more amino acid replacements, deletions or blocking mutations are made in sites 427-437, 367-398 and 257-297 of said amino acid sequence. Recombinant plasmid that determines synthesis of modified pneumolysine is constructed by vector pKK233-2 and comprises ampicillin- and tetracycline-resistant genes. Isopropyl beta-D- thiogalactopyranoside regulated promoter trc. Ribosom lac binding site adjacent to initiation codon ATG. Restriction sites for endonulceases Pst I and Hind III. Modified pneumolysine gene cloned into NCO I site which has certain sequence of nucleotides. Vaccine capable of inducing immune response in animals comprises antigen from modified pneumolysine. EFFECT: improved properties of the vaccine. 7 cli
|
申请公布号 |
RU2121481(C1) |
申请公布日期 |
1998.11.10 |
申请号 |
SU19894895772 |
申请日期 |
1989.12.15 |
申请人 |
ISTR VAN VELZEJN, VOLKSGEZONDKHEJD EHN KJULTJUR;STAT DER NEDERLANDEN VERTEGENV |
发明人 |
DZHEJMS KLILEND PEJTON;DEHVID DZHON KHEHNSMEN;GREHKHEM DZHON BAULNOJS;PITER VILL'EM EHNDRJU;TIMOTI DZHON MITCHELL;DZHON ARTUR VOKER |
分类号 |
C12N15/09;A61K31/715;A61K38/00;A61K39/00;A61K39/09;A61P11/00;A61P29/00;A61P31/04;C07K14/315;C07K14/37;C07K14/41;C12N1/21;C12N15/31;C12P21/02;C12R1/19;C12R1/46;(IPC1-7):C07K14/315 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|