发明名称 |
Crystalline chondroitinase isolated from Proteus vulgaris ATCC 6896 |
摘要 |
A crystallizable, purified chondroitinase ABC having a molecular weight of about 100,000 dalton by the measurement of the SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and the measurement by the gel permeation chromatography method, having alanine as the N-terminal amino acid and proline as the C-terminal amino acid is disclosed. A process for the purification of the crystallizable purified chondroitinase ABC comprising removing nucleic acid from an surfactant solution extract obtained from cells of chondroitinase ABC-producing microorganisms and chromatographically treating by concentration gradient elution using a weak cation exchange resin or a strong cation exchange resin is disclosed. A composition comprising a chondroitinase and serum albumin, gelatin, or a nonionic surfactant is disclosed. The enzyme is isolated from Proteus vulgaris ATCC 6896.
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申请公布号 |
US5773277(A) |
申请公布日期 |
1998.06.30 |
申请号 |
US19950445206 |
申请日期 |
1995.05.18 |
申请人 |
SEIKAGAKU KOGYO KABUSHIKI KAISHA |
发明人 |
HASHIMOTO, NOBUKAZU;MOCHIZUKI, HIDEO |
分类号 |
C12N9/88;(IPC1-7):C12N9/88;C12N9/44 |
主分类号 |
C12N9/88 |
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