发明名称 |
DNA CONSTRUCT, A TRANSFORMED CELL AND METHOD FOR PRODUCING AN EXTRANEOUS PROTEIN IN THE TRANSFORMED CELL |
摘要 |
A method for producing a foreign protein in a host organism whereby the protein is processed through the secretory pathway of the host. Production and processing is achieved by transforming the host organism with a vector comprising at least the signal peptide coding region of the yeast S. cerevisiae BAR1 gene fused to a foreign structural gene. DNA constructs and transformants are also provided containing a region of the BAR1 gene encoding the signal peptide and a KEX2 processing site, and a foreign structural gene. In particular, proinsulin is expressed and secreted from Schizosaccharomyces pombe and Saccharomyces cerevisiae. |
申请公布号 |
SK776486(A3) |
申请公布日期 |
1998.06.03 |
申请号 |
SK19860007764 |
申请日期 |
1986.10.27 |
申请人 |
ZYMOGENETICS, INC. |
发明人 |
MACKAY VIVIAN L. |
分类号 |
C12N15/00;C07K14/575;C07K14/62;C07K19/00;C12N1/14;C12N1/15;C12N1/16;C12N1/18;C12N1/19;C12N9/60;C12N15/09;C12N15/62;C12N15/81;C12P21/02;C12R1/645;C12R1/865 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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