| 摘要 |
<p>The present invention relates generally to the field of molecular biology and specifically to rapid, high-throughput gene discovery methods that facilitate genome closure and to methods for analyzing gene expression patterns. The present invention provides methods and vectors useful for constructing libraries of transcript markers. The present invention also provides sequence specific methods for extending the nucleotide sequence of partial transcripts in a high-throughput manner using polymerase chain reaction.</p> |
