发明名称 |
Stable DNA constructs |
摘要 |
Methods are provided for producing protein products in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is s strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selection marker whereby the growth conditions for selection may comprise a conventional complex medium.
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申请公布号 |
US5723292(A) |
申请公布日期 |
1998.03.03 |
申请号 |
US19950488179 |
申请日期 |
1995.06.07 |
申请人 |
ZYMOGENETICS, INC. |
发明人 |
KAWASAKI, GLENN H.;BELL, LESLIE;IRANI, MEHER |
分类号 |
C07K14/15;C07K14/49;C07K14/81;C12N9/90;C12N15/64;(IPC1-7):C12P21/02 |
主分类号 |
C07K14/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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