发明名称 VNTR probes and methods of using thereof
摘要 According to the process of the invention, sets of restriction fragments from the DNA of an individual are prepared, these restriction fragments being separated by size. Probes are prepared by enzymatic hydrolysis of DNA from a genome bank of the species to which this individual belongs, separation of the fragments obtained as a function of their size, labeling these probes and placing them in contact, under hybridization conditions, with the aforementioned sets of restriction fragments. Finally, selection is made of the probes (capable of hybridizing with said set of restriction fragments) which do not give hybridization profiles identical to those obtained with known probes recognizing variable number tandem repeat regions. Applications for the probes thus obtained include, particularly, processes for identifying an individual, consanguinity testing, and investigating the origin of a seed.
申请公布号 US5716786(A) 申请公布日期 1998.02.10
申请号 US19960690129 申请日期 1996.07.31
申请人 L'ETAT FRANCAIS, REPRESENTE PAR LE DELEGUE MINISTERIEL POUR L'ARMEMENT 发明人 BUARD, JEROME;GAUGUIER, DOMINIQUE;VERGNAUD, GILLES
分类号 C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04;C07H21/02 主分类号 C12Q1/68
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