发明名称 |
A RECOMBINANT ENZYME WITH MUTANASE ACTIVITY |
摘要 |
The present invention relates to method for constructing an expression vector comprising a mutanase gene obtained from a filamentous fungus suitable for heterologous production comprising the steps of a) isolating a DNA sequence encoding a mutanase from a filamentous fungus, b) introducing a kex2-site or kex2-like site between the DNA sequences encoding the pro-peptide and the mature region of the mutanase, c) cloning the DNA sequence obtained in step b) into a suitable expression vector. The invention also relates to a recombinant expression vector comprising said mutanase gene sequence and a kex2 cleavage site between the DNA sequence encoding the pro-peptide and the region encoding the mature mutanase, a filamentous fungus host cell, a process for producing recombinant mutanase and a recombinant mutanase. It is also the object of the invention to provide compositions useful in oral care products for humans and animals. |
申请公布号 |
CA2258291(A1) |
申请公布日期 |
1998.01.08 |
申请号 |
CA19972258291 |
申请日期 |
1997.06.30 |
申请人 |
NOVO NORDISK A/S |
发明人 |
HANSEN, MOGENS TRIER;JOHANSEN, CHARLOTTE;FUGLSANG, CLAUS CRONE;WAHLEITHNER, JILL ANGELA;HALKIER, TORBEN |
分类号 |
A23K1/165;A23L1/03;A61K8/00;A61K8/66;A61K8/99;A61K38/00;A61Q11/00;C12N1/15;C12N9/24;C12N15/09;C12N15/56;C12R1/69;C12R1/885 |
主分类号 |
A23K1/165 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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