A CELL-FREE SYSTEM FOR INITIATION OF DNA REPLICATION
摘要
A cell-free system for initiating DNA replication under cell cycle control includes S phase cytosol or a fraction thereof in which are incubated G1 phase nuclei, which are co-incubated with S phase nuclei or a fraction thereof and/or cyclins A and/or E complexed to their cognate cyclin dependent kinase (Cdk2). The system may be used to assay for substances which modulate DNA synthesis or initiation thereof, and which have therapeutic potential in a number of contexts.
申请公布号
WO9749797(A1)
申请公布日期
1997.12.31
申请号
WO1997GB01751
申请日期
1997.06.26
申请人
CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED;LASKEY, RONALD, ALFRED;KRUDE, TORSTEN;JACKMAN, MARK, RICHARD;PINES, JONATHAN, NOE, JOSEPH
发明人
LASKEY, RONALD, ALFRED;KRUDE, TORSTEN;JACKMAN, MARK, RICHARD;PINES, JONATHAN, NOE, JOSEPH