| 摘要 |
PCT No. PCT/SE93/00854 Sec. 371 Date Apr. 18, 1995 Sec. 102(e) Date Apr. 18, 1995 PCT Filed Oct. 19, 1993 PCT Pub. No. WO94/09122 PCT Pub. Date Apr. 28, 1994The invention relates to an enzyme, exo- alpha -1,4-glucan lyase, capable of successively cleaving the terminal alpha -1,4-D-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan. The enzyme is selected from the group consisting of alpha -1,4-glucan lyase isolated from an alga and functional derivatives and analogs derived from a nucleotide sequence related to an alga alpha -1,4-glucan lyase. Further, a method of enzymatically cleaving the terminal alpha -1,4-D-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan, and the degradation product 1,5-anhydrofructose for use as an oxygen radical scavenger or anti-oxidant and/or as a sugar substitute, are disclosed. Additionally, a method of producing an enzyme capable of successively cleaving the terminal alpha -1,4-glucosidic bonds from the non-reducing ends of an alpha -1,4-glucan; an antibody which binds to the amino acid sequence of the enzyme, and a DNA or RNA probe which recognizes a nucleotide sequence coding for the enzyme, are described.
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