<p>Sequencing of a selected region of a target nucleic acid polymer in a natural abundance DNA sample can be performed in a single vessel by combining the sample with a sequencing mixture containing a primer pair, a thermally stable polymerase such as ThermoSequenaseTM which incorporates dideoxynucleotides into an extending nucleic acid polymer at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleotides, nucleotide feedstocks, and a chain terminating nucleotide. The mixture is processed through multiple thermal cycles for annealing, extension and denaturation to produce a product mixture which is analyzed by electrophoresis.</p>
申请公布号
CA2252588(A1)
申请公布日期
1997.11.06
申请号
CA19972252588
申请日期
1997.04.29
申请人
LACROIX, JEAN-MICHEL;VISIBLE GENETICS INC.;SHIPMAN, ROBERT;DUNN, JAMES M.;LEUSHNER, JAMES;HUI, MAY
发明人
LACROIX, JEAN-MICHEL;LARSON, MARINA T.;SHIPMAN, ROBERT;DUNN, JAMES M.;LEUSHNER, JAMES;HUI, MAY