WHOLE BLOOD SAMPLE PREPARATION FOR POLYMERASE CHAIN REACTION USING AMMONIUM CHLORIDE AND A CARBOXYLIC ACID OR METAL CARBOXYLATE FOR SELECTIVE RED BLOOD CELL LYSIS

摘要

Leucocytes can be quickly and selectively separated from erythrocytes by subjecting a whole blood sample to a series of steps including lysing the erythrocytes and washing the remaining leucocytes with a solution containing ammonium chloride and a carboxylic acid or a metal carboxylate. The resulting white blood cells can be readily lysed and subjected to polymerase chain reaction to amplify and detect a target nucleic acid. The test kit useful in practicing the amplification method includes a labeled primer, a PCR reagent and a reagent mixture containing ammonium chloride and a carboxylic acid or a metal carboxylate for sample preparation.