发明名称 |
Compositions and methods for detection of 2,4-dichlorophenoxyacetic acid and related compounds |
摘要 |
Methods and compositions are provided for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) and other phenoxy ether compounds. The phenoxy ether bond of 2,4-D is enzymatically cleaved by 2,4-D alpha -ketoglutarate dioxygenase to form 2,4-dichlorophenol, which is assayed by the 4-aminoantipyrine method. The enzyme is supplied in a dried form, preferably immobilized on a solid support, and is stable at room temperature for several months even in a highly impure state, e.g., crude cell extracts or dried cells.
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申请公布号 |
US5656422(A) |
申请公布日期 |
1997.08.12 |
申请号 |
US19940318505 |
申请日期 |
1994.10.05 |
申请人 |
IDAHO RESEARCH FOUNDATION, INC. |
发明人 |
CRAWFORD, RONALD L.;GU, YONGXIANG;KORUS, ROGER A.;KNAEBEL, DAVID B. |
分类号 |
C12N9/02;C12Q1/26;(IPC1-7):C12Q1/00;C12N5/00 |
主分类号 |
C12N9/02 |
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