摘要 |
FIELD: medicine. SUBSTANCE: method involves milling cattle spleen, extraction, extract separation and extractant removing. Ethyl alcohol aqueous solution is used as an extractant. Extraction is carried out at simultaneous effect by low-frequency ultrasonic treatment. Extractant is separated by centrifugation. Extractant is preferably removed by alcohol distillation off under vacuum at 45 C, not above. An aqueous extract can be lyophilized. Extractant can contain 65-75% ethanol at weight ratio of spleen and extractant = 1/6-1/3. Ultrasonic frequency is at the range 18-70 kHz at intensity 0.1-2.3 W/cm.. EFFECT: increased yield of the end product, decreased process time. 8 cl, 3 tbl |