IgG SEPARATION MEDIUM AND NOVEL PROTEIN A VARIANT

摘要

A separation medium comprising a base matrix and matrix-bound groups which exhibit recombinant Protein A containing a cysteine. The groups comply with the formula: - B - X - rProtein A-cys where B is a bridge which binds to the base matrix and X includes a heteroatom N or S from rProtein A-cys. The characterizing feature is that X is thioether sulphur and/or a secondary amine (-NH-). Protein A variant characterized in that the C-terminal residue is cysteine.