The present invention relates to mutants of D-N- alpha -carbamylase wherein at least one of the cysteines in position 243, 250 and 279 of the amino acid sequence of the wild type enzyme is substituted with a different residue selected from natural amino acids; a recombinant plasmid comprising a nucleotidic sequence which encodes for at least one of the mutants of D-N- alpha -carbamylase, host microorganisms transformed with said plasmid and a process for the preparation of these mutants by the culture of said microorganisms. The mutants of D-N- alpha -carbamylase have a higher enzymatic stability than that of the wild type enzyme and are particularly useful in the preparation of D- alpha -amino acids providing an improvement in the production yields. <IMAGE>
申请公布号
DE69500108(D1)
申请公布日期
1997.01.23
申请号
DE1995600108
申请日期
1995.03.13
申请人
ENIRICERCHE S.P.A., SAN DONATO MILANESE, MAILAND/MILANO, IT
发明人
GIULIANO, GALLI, SAN DONATO MIL.SE, IT;RENATA, GRIFANTINI, MILANO, IT;GUIDO, GRANDI, SEGRATE, SAN FELICE, IT