| 摘要 |
PURPOSE: To accurately measure the amount of VPF(vascular permeability factor) by using the chemiluminescence as a detection method of enzyme immunity measurement using anti-VPF polyclonal antibody. CONSTITUTION: Anti-VPF polyclonal antibody (5μg/ml) is poured into each hole of 96-hole plate, is left stand for one night at approximately 4 deg.C, is washed in phosphoric acid buffering physiological salt solution containing 0.1% bovine serum albumin(BSA), and is blocked for approximately one hour in 1% BSA- containing buffer solution. The serum of a healthy person which is diluted approximately ten times by sodium phosphate buffer solution containing 1% BSA is added and is left stand for approximately one hour. It is washed with tris buffer solution, alkali phosphatase labeled antibody VPF polyclonal antibody is added to react for approximately one hour, and is washed with tris buffer liquid. For example, Lumi-Phos 530 which is diluted by approximately five times in a buffer solution is added into it, and the entire part is left stand for approximately 30 minutes at approximately 37 deg.C. After that, by measuring luminous intensity, a small amount of VPF in the humor derived from a human can be measured.
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