IN VITRO DIFFERENTIATION OF CD34<+> PROGENITOR CELLS INTO T LYMPHOCYTES
摘要
The invention involves a method for the in vitro T cell production. A monolayer of non-human primate thymic stromal cells are cocultured in vitro with primate hematopoietic T cell progenitor cells. This results in the differentiation and growth of mature T cells. The T cells may be isolated at any sequential stage of differentiation and further expanded by coculture with a mitogenic agent. The T cells also may be genetically altered at any stage of the process. The effect of agents on the growth and differentiation of T cells may be measured by comparing a coculture containing the agent with a control coculture and comparing the differentiation or growth of the T cells progenitor cells in the test culture with the control culture. Kits and novel populations of T cells are provided.
申请公布号
WO9633265(A1)
申请公布日期
1996.10.24
申请号
WO1996US05607
申请日期
1996.04.22
申请人
PRESIDENT AND FELLOWS OF HARVARD COLLEGE;NEW ENGLAND DEACONESS HOSPITAL CORPORATION
发明人
JOHNSON, R., PAUL;ROSENZWEIG, MICHAEL;SCADDEN, DAVID, T.