| 摘要 |
PCT No. PCT/EP92/01873 Sec. 371 Date May 24, 1994 Sec. 102(e) Date May 24, 1994 PCT Filed Aug. 17, 1992 PCT Pub. No. WO93/12235 PCT Pub. Date Jun. 24, 1993Engineered DNA encoding 3-phosphoglycerate dehydrogenate (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells. |
