CAPILLARY-BASED SEPARATION METHODS FOR IDENTIFYING BIOACTIVE ANALYTES IN A MIXTURE

摘要

Methods for detecting and methods of assaying the bioactivity of compounds and ligands, separated by capillary-based separation, such as capillary electrophoresis, from a mixture, employing an intact cell biosensor loaded with a calcium dye indicator for monitoring the bioactivity of said compounds and ligands. The figure shows an exemplary apparatus for recording optical signals from cell biosensors. Labelled parts are as follows: Number (2) functions to capillary separate ligands introduced at Number (4) the inlet end from a mixture of ligands contained in Number (6), Number (20) a power supply, (22) and (24) are electrodes, Number (16) an XYZ positioner, Number (18) a hollow needle through which the capillary is threaded into the recording chamber Number (14), the location where the response of a dye is detected via an optical detector, Number (28).